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clone rea749  (Miltenyi Biotec)


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    Miltenyi Biotec clone rea749
    Clone Rea749, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clone+rea749/CD19-PE-Vio770/pm35613582-241-58-60
    Average 95 stars, based on 53 article reviews
    clone rea749 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunopeptidomics:

    Article Title: Mitochondrial damage-associated molecular patterns trigger arginase-dependent lymphocyte immunoregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human CD56 PE-Vio615 (Clone REA196) Miltenyi Biotec Cat # 130-114-550; RRID: AB_2726696 Human CD56 FITC (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726695 Human CD56 PE (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726090 Human CD56 PerCP-Vio 700 (Cone REA196) Miltenyi Biotec Cat # 130-114-551; RRID: AB_2733038 Human CD3 (CD3ε) APC (Clone REA613) Miltenyi Biotec Cat # 130-113-697; RRID: AB_2726238 Human CD3 (CD3ε) BV650 (Clone UCHT1) BD Biosciences Cat # 563851; RRID: AB_2744391 Human CD8 BV650 (Clone RPA-T8) BD Biosciences Cat # 563821; RRID: AB_2744462 Human CD4 PerCP-Cy5.5 (Clone SK3 [also known as Leu3a]) BD Biosciences Cat # 566321; RRID: AB_2739683 Human CD69 AF700 (Clone FN50) BD Biosciences Cat # 560739; RRID: AB_1727505 Human CD69 BV421 (Clone FN50) BD Biosciences Cat # 562884; RRID: AB_2687422 Human IFN-g (gamma) BV711 (Clone B27) BD Biosciences Cat # 564039; RRID: AB_2738557 Human CD107a (LAMP-1) (Clone H4A3) BD Biosciences Cat # 561343; RRID: AB_10644020 Human PD-L1 (CD273, B7-H1) BV421 (Clone MIH1) BD Biosciences Cat # 563738; RRID: AB_2738396 Human CD73 PE (Clone REA804) Miltenyi Biotec Cat # 130-112-060; RRID: AB_2659164 Human NKG2D (CD314) PE (Clone 1D11) BD Bioscience Cat # 557940; RRID: AB_396951 Human CD25 PE-Cy7 (Clone M-A251) BD Biosciences Cat # 557741; RRID: AB_396847 Human FoxP3 APC (Clone FJK-16s) Thermo Fisher Scientific Cat # 17-5773-82; RRID: AB_469457 Human purified anti-IL-10 (Clone JES3-9D7) BioLegend Cat # 501401; RRID: AB_315167 Human purified anti-IL-10R (Clone 3F9) BioLegend Cat # 308802; RRID: AB_314734 Isotype control (for human IL-10) (Clone eBRG1) BioLegend Cat # 16-4301-85; RRID: AB_470154 Isotype control (for human IL-10R) (Clone RTK2758) Thermo Fisher Scientific Cat # 400516; RRID: AB_2861020 Mouse CD3 FITC (Clone REA641) Miltenyi Biotec Cat # 130-119-798; RRID: AB_2751851 Mouse CD73 AF647 (Clone TY/23) BD Biosciences Cat # 561543; RRID: AB_10896329 Mouse CD69 APC-efluor780 (Clone H1.2F3) Thermo Fisher Scientific Cat # 47-0691-82; RRID: AB_2573966 Mouse NK1.1 APC (Clone PK136) BioLegend Cat # 108710; RRID: AB_313397 Mouse NK1.1 PerCP-Vio700 (Clone PK136) Miltenyi Biotec Cat # 130-117-773; RRID: AB_2728038 (Continued on next page) Cell Reports 39, 110847, May 24, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse NK1.1 PerCP/Cyanine 5.5 (Clone PK136) BioLegend Cat # 108727; RRID: AB_2132706 Mouse CD11c APC-eFluor 780 (Clone N418) Thermo Fisher Scientific Cat # 47-0114-82; RRID: AB_1548652 Mouse CD11b BV650 (Clone M1/70) BD Biosciences Cat # 563402; RRID: AB_2738184 Mouse Ly6c PerCP/Cyanine 5.5 (Clone HK1.4) Biolegend Cat # 128012; RRID: AB_1659241 Mouse CD19 PE-Vio770 (Clone REA749) Miltenyi Biotec Cat # 130-111-885; RRID: AB_2655831 Mouse CD86 BV421 (Clone GL1) BD Biosciences Cat # 564198; RRID: AB_2738663 Mouse MHC II (I-Ab) biotin (Clone 25-9-17) BD Biosciences Cat # 553604; RRID: AB_394948 Mouse MHC II (I-A/I-E) (Clone M5/114.15.2) Thermo Fisher Scientific Cat # 56-5321-82; RRID: AB_494009 Mouse CD8a BV605 (Clone 53–6.7) BioLegend Cat # 100744; RRID: AB_2562609 Mouse IFN-gamma BV650 (Clone XMG1.2) BioLegend Cat # 505831; RRID: AB_11142685 Mouse IFN-gamma PE-CF594 (Clone XMG1.2) BD Biosciences Cat # 562303; RRID: AB_11153140 Mouse CD45.1 PerCP-Cy5.5 (Clone A20) Thermo Fisher Scientific Cat # 45-0453-82; RRID: AB_1107003 Mouse PD-L1 (CD274, B7-H1) PE (Clone 10F.9G2) BioLegend Cat # 124308; RRID: AB_2073556 Mouse CD107a (LAMP-1) APC (Clone 1D4B) Biolegend Cat # 121613; RRID: AB_2134486 Mouse Perforin PE (Clone S16009B) BioLegend Cat # 154405; RRID: AB_2721640 Mouse TLR4 (CD284) PE (Clone SA15-21) BioLegend Cat #: 145403; RRID: AB_2561873 Mouse TLR9 (CD289) FITC (Clone M9.D6) Thermo Fisher Scientific Cat # 11-9093-80; RRID: AB_465443 Bacterial and virus strains VSV-Dm51 Dr. Douglas Mahoney N/A Biological samples Healthy human peripheral blood mononuclear cells Canadian Blood Services Blood4Research program Chemicals, peptides, and recombinant proteins Protein Assay Dye Reagent Concentrate Bio-Rad Cat # 5000006 Pierce Chromogenic Endotoxin Quant Kit Thermo Fisher Scientific Cat # A39552 NK MACS Medium (human NK cell expansion medium) Miltenyi Biotec Cat # 130-114-429 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat # 2430054 Roswell Park Memorial Institute 1649 Medium (RPMI) Thermo Fisher Scientific Cat # 11875093 HEPES buffer Thermo Fisher Scientific Cat # 15630106 Minimum Essential Medium a (MEM-a) Thermo Fisher Scientific Cat # 12571063 Horse Serum, heat inactivated Thermo Fisher Scientific Cat # 26050070 Myo-Inositol Milipore Sigma Cat # I7508 b-mercaptoethanol Thermo Fisher Scientific Cat # 21985023 Folic acid Milipore Sigma Cat # F8758 Sodium pyruvate Thermo Fisher Scientific Cat # 25000CI (Continued on next page) e2 Cell Reports 39, 110847, May 24, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: organisms/strains Mouse: C57BL/6NCrl Charles River Laboratories https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Mouse: IL-10-IRES-eGFP knock-in (IL-10GFP or tiger [interleukin-ten ires gfo-enhanced reporter]) Dr. Richard A. Flavell, via Dr. Jun Wang https://doi.org/10.1016/j.immuni.2006.09.013 Oligonucleotides TLR 9 agonist CpG DNA Invivogen Cat # tlrl-1668 Software and algorithms FlowJo Version 10.7.1 BD Biosciences https://www.flowjo.com/ Prism Version 9.1.2 GraphPad https://www.graphpad.com/scientific-software/ prism/ Reactome Pathway Browser 3.7 Reactome https://reactome.org/; RRID: SCR_003485 Uniprot Universal Protein Resource https://www.uniprot.org/; RRID: SCR_002380

    Virus:

    Article Title: Mitochondrial damage-associated molecular patterns trigger arginase-dependent lymphocyte immunoregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human CD56 PE-Vio615 (Clone REA196) Miltenyi Biotec Cat # 130-114-550; RRID: AB_2726696 Human CD56 FITC (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726695 Human CD56 PE (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726090 Human CD56 PerCP-Vio 700 (Cone REA196) Miltenyi Biotec Cat # 130-114-551; RRID: AB_2733038 Human CD3 (CD3ε) APC (Clone REA613) Miltenyi Biotec Cat # 130-113-697; RRID: AB_2726238 Human CD3 (CD3ε) BV650 (Clone UCHT1) BD Biosciences Cat # 563851; RRID: AB_2744391 Human CD8 BV650 (Clone RPA-T8) BD Biosciences Cat # 563821; RRID: AB_2744462 Human CD4 PerCP-Cy5.5 (Clone SK3 [also known as Leu3a]) BD Biosciences Cat # 566321; RRID: AB_2739683 Human CD69 AF700 (Clone FN50) BD Biosciences Cat # 560739; RRID: AB_1727505 Human CD69 BV421 (Clone FN50) BD Biosciences Cat # 562884; RRID: AB_2687422 Human IFN-g (gamma) BV711 (Clone B27) BD Biosciences Cat # 564039; RRID: AB_2738557 Human CD107a (LAMP-1) (Clone H4A3) BD Biosciences Cat # 561343; RRID: AB_10644020 Human PD-L1 (CD273, B7-H1) BV421 (Clone MIH1) BD Biosciences Cat # 563738; RRID: AB_2738396 Human CD73 PE (Clone REA804) Miltenyi Biotec Cat # 130-112-060; RRID: AB_2659164 Human NKG2D (CD314) PE (Clone 1D11) BD Bioscience Cat # 557940; RRID: AB_396951 Human CD25 PE-Cy7 (Clone M-A251) BD Biosciences Cat # 557741; RRID: AB_396847 Human FoxP3 APC (Clone FJK-16s) Thermo Fisher Scientific Cat # 17-5773-82; RRID: AB_469457 Human purified anti-IL-10 (Clone JES3-9D7) BioLegend Cat # 501401; RRID: AB_315167 Human purified anti-IL-10R (Clone 3F9) BioLegend Cat # 308802; RRID: AB_314734 Isotype control (for human IL-10) (Clone eBRG1) BioLegend Cat # 16-4301-85; RRID: AB_470154 Isotype control (for human IL-10R) (Clone RTK2758) Thermo Fisher Scientific Cat # 400516; RRID: AB_2861020 Mouse CD3 FITC (Clone REA641) Miltenyi Biotec Cat # 130-119-798; RRID: AB_2751851 Mouse CD73 AF647 (Clone TY/23) BD Biosciences Cat # 561543; RRID: AB_10896329 Mouse CD69 APC-efluor780 (Clone H1.2F3) Thermo Fisher Scientific Cat # 47-0691-82; RRID: AB_2573966 Mouse NK1.1 APC (Clone PK136) BioLegend Cat # 108710; RRID: AB_313397 Mouse NK1.1 PerCP-Vio700 (Clone PK136) Miltenyi Biotec Cat # 130-117-773; RRID: AB_2728038 (Continued on next page) Cell Reports 39, 110847, May 24, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse NK1.1 PerCP/Cyanine 5.5 (Clone PK136) BioLegend Cat # 108727; RRID: AB_2132706 Mouse CD11c APC-eFluor 780 (Clone N418) Thermo Fisher Scientific Cat # 47-0114-82; RRID: AB_1548652 Mouse CD11b BV650 (Clone M1/70) BD Biosciences Cat # 563402; RRID: AB_2738184 Mouse Ly6c PerCP/Cyanine 5.5 (Clone HK1.4) Biolegend Cat # 128012; RRID: AB_1659241 Mouse CD19 PE-Vio770 (Clone REA749) Miltenyi Biotec Cat # 130-111-885; RRID: AB_2655831 Mouse CD86 BV421 (Clone GL1) BD Biosciences Cat # 564198; RRID: AB_2738663 Mouse MHC II (I-Ab) biotin (Clone 25-9-17) BD Biosciences Cat # 553604; RRID: AB_394948 Mouse MHC II (I-A/I-E) (Clone M5/114.15.2) Thermo Fisher Scientific Cat # 56-5321-82; RRID: AB_494009 Mouse CD8a BV605 (Clone 53–6.7) BioLegend Cat # 100744; RRID: AB_2562609 Mouse IFN-gamma BV650 (Clone XMG1.2) BioLegend Cat # 505831; RRID: AB_11142685 Mouse IFN-gamma PE-CF594 (Clone XMG1.2) BD Biosciences Cat # 562303; RRID: AB_11153140 Mouse CD45.1 PerCP-Cy5.5 (Clone A20) Thermo Fisher Scientific Cat # 45-0453-82; RRID: AB_1107003 Mouse PD-L1 (CD274, B7-H1) PE (Clone 10F.9G2) BioLegend Cat # 124308; RRID: AB_2073556 Mouse CD107a (LAMP-1) APC (Clone 1D4B) Biolegend Cat # 121613; RRID: AB_2134486 Mouse Perforin PE (Clone S16009B) BioLegend Cat # 154405; RRID: AB_2721640 Mouse TLR4 (CD284) PE (Clone SA15-21) BioLegend Cat #: 145403; RRID: AB_2561873 Mouse TLR9 (CD289) FITC (Clone M9.D6) Thermo Fisher Scientific Cat # 11-9093-80; RRID: AB_465443 Bacterial and virus strains VSV-Dm51 Dr. Douglas Mahoney N/A Biological samples Healthy human peripheral blood mononuclear cells Canadian Blood Services Blood4Research program Chemicals, peptides, and recombinant proteins Protein Assay Dye Reagent Concentrate Bio-Rad Cat # 5000006 Pierce Chromogenic Endotoxin Quant Kit Thermo Fisher Scientific Cat # A39552 NK MACS Medium (human NK cell expansion medium) Miltenyi Biotec Cat # 130-114-429 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat # 2430054 Roswell Park Memorial Institute 1649 Medium (RPMI) Thermo Fisher Scientific Cat # 11875093 HEPES buffer Thermo Fisher Scientific Cat # 15630106 Minimum Essential Medium a (MEM-a) Thermo Fisher Scientific Cat # 12571063 Horse Serum, heat inactivated Thermo Fisher Scientific Cat # 26050070 Myo-Inositol Milipore Sigma Cat # I7508 b-mercaptoethanol Thermo Fisher Scientific Cat # 21985023 Folic acid Milipore Sigma Cat # F8758 Sodium pyruvate Thermo Fisher Scientific Cat # 25000CI (Continued on next page) e2 Cell Reports 39, 110847, May 24, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: organisms/strains Mouse: C57BL/6NCrl Charles River Laboratories https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Mouse: IL-10-IRES-eGFP knock-in (IL-10GFP or tiger [interleukin-ten ires gfo-enhanced reporter]) Dr. Richard A. Flavell, via Dr. Jun Wang https://doi.org/10.1016/j.immuni.2006.09.013 Oligonucleotides TLR 9 agonist CpG DNA Invivogen Cat # tlrl-1668 Software and algorithms FlowJo Version 10.7.1 BD Biosciences https://www.flowjo.com/ Prism Version 9.1.2 GraphPad https://www.graphpad.com/scientific-software/ prism/ Reactome Pathway Browser 3.7 Reactome https://reactome.org/; RRID: SCR_003485 Uniprot Universal Protein Resource https://www.uniprot.org/; RRID: SCR_002380

    Recombinant:

    Article Title: Mitochondrial damage-associated molecular patterns trigger arginase-dependent lymphocyte immunoregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human CD56 PE-Vio615 (Clone REA196) Miltenyi Biotec Cat # 130-114-550; RRID: AB_2726696 Human CD56 FITC (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726695 Human CD56 PE (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726090 Human CD56 PerCP-Vio 700 (Cone REA196) Miltenyi Biotec Cat # 130-114-551; RRID: AB_2733038 Human CD3 (CD3ε) APC (Clone REA613) Miltenyi Biotec Cat # 130-113-697; RRID: AB_2726238 Human CD3 (CD3ε) BV650 (Clone UCHT1) BD Biosciences Cat # 563851; RRID: AB_2744391 Human CD8 BV650 (Clone RPA-T8) BD Biosciences Cat # 563821; RRID: AB_2744462 Human CD4 PerCP-Cy5.5 (Clone SK3 [also known as Leu3a]) BD Biosciences Cat # 566321; RRID: AB_2739683 Human CD69 AF700 (Clone FN50) BD Biosciences Cat # 560739; RRID: AB_1727505 Human CD69 BV421 (Clone FN50) BD Biosciences Cat # 562884; RRID: AB_2687422 Human IFN-g (gamma) BV711 (Clone B27) BD Biosciences Cat # 564039; RRID: AB_2738557 Human CD107a (LAMP-1) (Clone H4A3) BD Biosciences Cat # 561343; RRID: AB_10644020 Human PD-L1 (CD273, B7-H1) BV421 (Clone MIH1) BD Biosciences Cat # 563738; RRID: AB_2738396 Human CD73 PE (Clone REA804) Miltenyi Biotec Cat # 130-112-060; RRID: AB_2659164 Human NKG2D (CD314) PE (Clone 1D11) BD Bioscience Cat # 557940; RRID: AB_396951 Human CD25 PE-Cy7 (Clone M-A251) BD Biosciences Cat # 557741; RRID: AB_396847 Human FoxP3 APC (Clone FJK-16s) Thermo Fisher Scientific Cat # 17-5773-82; RRID: AB_469457 Human purified anti-IL-10 (Clone JES3-9D7) BioLegend Cat # 501401; RRID: AB_315167 Human purified anti-IL-10R (Clone 3F9) BioLegend Cat # 308802; RRID: AB_314734 Isotype control (for human IL-10) (Clone eBRG1) BioLegend Cat # 16-4301-85; RRID: AB_470154 Isotype control (for human IL-10R) (Clone RTK2758) Thermo Fisher Scientific Cat # 400516; RRID: AB_2861020 Mouse CD3 FITC (Clone REA641) Miltenyi Biotec Cat # 130-119-798; RRID: AB_2751851 Mouse CD73 AF647 (Clone TY/23) BD Biosciences Cat # 561543; RRID: AB_10896329 Mouse CD69 APC-efluor780 (Clone H1.2F3) Thermo Fisher Scientific Cat # 47-0691-82; RRID: AB_2573966 Mouse NK1.1 APC (Clone PK136) BioLegend Cat # 108710; RRID: AB_313397 Mouse NK1.1 PerCP-Vio700 (Clone PK136) Miltenyi Biotec Cat # 130-117-773; RRID: AB_2728038 (Continued on next page) Cell Reports 39, 110847, May 24, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse NK1.1 PerCP/Cyanine 5.5 (Clone PK136) BioLegend Cat # 108727; RRID: AB_2132706 Mouse CD11c APC-eFluor 780 (Clone N418) Thermo Fisher Scientific Cat # 47-0114-82; RRID: AB_1548652 Mouse CD11b BV650 (Clone M1/70) BD Biosciences Cat # 563402; RRID: AB_2738184 Mouse Ly6c PerCP/Cyanine 5.5 (Clone HK1.4) Biolegend Cat # 128012; RRID: AB_1659241 Mouse CD19 PE-Vio770 (Clone REA749) Miltenyi Biotec Cat # 130-111-885; RRID: AB_2655831 Mouse CD86 BV421 (Clone GL1) BD Biosciences Cat # 564198; RRID: AB_2738663 Mouse MHC II (I-Ab) biotin (Clone 25-9-17) BD Biosciences Cat # 553604; RRID: AB_394948 Mouse MHC II (I-A/I-E) (Clone M5/114.15.2) Thermo Fisher Scientific Cat # 56-5321-82; RRID: AB_494009 Mouse CD8a BV605 (Clone 53–6.7) BioLegend Cat # 100744; RRID: AB_2562609 Mouse IFN-gamma BV650 (Clone XMG1.2) BioLegend Cat # 505831; RRID: AB_11142685 Mouse IFN-gamma PE-CF594 (Clone XMG1.2) BD Biosciences Cat # 562303; RRID: AB_11153140 Mouse CD45.1 PerCP-Cy5.5 (Clone A20) Thermo Fisher Scientific Cat # 45-0453-82; RRID: AB_1107003 Mouse PD-L1 (CD274, B7-H1) PE (Clone 10F.9G2) BioLegend Cat # 124308; RRID: AB_2073556 Mouse CD107a (LAMP-1) APC (Clone 1D4B) Biolegend Cat # 121613; RRID: AB_2134486 Mouse Perforin PE (Clone S16009B) BioLegend Cat # 154405; RRID: AB_2721640 Mouse TLR4 (CD284) PE (Clone SA15-21) BioLegend Cat #: 145403; RRID: AB_2561873 Mouse TLR9 (CD289) FITC (Clone M9.D6) Thermo Fisher Scientific Cat # 11-9093-80; RRID: AB_465443 Bacterial and virus strains VSV-Dm51 Dr. Douglas Mahoney N/A Biological samples Healthy human peripheral blood mononuclear cells Canadian Blood Services Blood4Research program Chemicals, peptides, and recombinant proteins Protein Assay Dye Reagent Concentrate Bio-Rad Cat # 5000006 Pierce Chromogenic Endotoxin Quant Kit Thermo Fisher Scientific Cat # A39552 NK MACS Medium (human NK cell expansion medium) Miltenyi Biotec Cat # 130-114-429 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat # 2430054 Roswell Park Memorial Institute 1649 Medium (RPMI) Thermo Fisher Scientific Cat # 11875093 HEPES buffer Thermo Fisher Scientific Cat # 15630106 Minimum Essential Medium a (MEM-a) Thermo Fisher Scientific Cat # 12571063 Horse Serum, heat inactivated Thermo Fisher Scientific Cat # 26050070 Myo-Inositol Milipore Sigma Cat # I7508 b-mercaptoethanol Thermo Fisher Scientific Cat # 21985023 Folic acid Milipore Sigma Cat # F8758 Sodium pyruvate Thermo Fisher Scientific Cat # 25000CI (Continued on next page) e2 Cell Reports 39, 110847, May 24, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: organisms/strains Mouse: C57BL/6NCrl Charles River Laboratories https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Mouse: IL-10-IRES-eGFP knock-in (IL-10GFP or tiger [interleukin-ten ires gfo-enhanced reporter]) Dr. Richard A. Flavell, via Dr. Jun Wang https://doi.org/10.1016/j.immuni.2006.09.013 Oligonucleotides TLR 9 agonist CpG DNA Invivogen Cat # tlrl-1668 Software and algorithms FlowJo Version 10.7.1 BD Biosciences https://www.flowjo.com/ Prism Version 9.1.2 GraphPad https://www.graphpad.com/scientific-software/ prism/ Reactome Pathway Browser 3.7 Reactome https://reactome.org/; RRID: SCR_003485 Uniprot Universal Protein Resource https://www.uniprot.org/; RRID: SCR_002380

    Magnetic Cell Separation:

    Article Title: Mitochondrial damage-associated molecular patterns trigger arginase-dependent lymphocyte immunoregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human CD56 PE-Vio615 (Clone REA196) Miltenyi Biotec Cat # 130-114-550; RRID: AB_2726696 Human CD56 FITC (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726695 Human CD56 PE (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726090 Human CD56 PerCP-Vio 700 (Cone REA196) Miltenyi Biotec Cat # 130-114-551; RRID: AB_2733038 Human CD3 (CD3ε) APC (Clone REA613) Miltenyi Biotec Cat # 130-113-697; RRID: AB_2726238 Human CD3 (CD3ε) BV650 (Clone UCHT1) BD Biosciences Cat # 563851; RRID: AB_2744391 Human CD8 BV650 (Clone RPA-T8) BD Biosciences Cat # 563821; RRID: AB_2744462 Human CD4 PerCP-Cy5.5 (Clone SK3 [also known as Leu3a]) BD Biosciences Cat # 566321; RRID: AB_2739683 Human CD69 AF700 (Clone FN50) BD Biosciences Cat # 560739; RRID: AB_1727505 Human CD69 BV421 (Clone FN50) BD Biosciences Cat # 562884; RRID: AB_2687422 Human IFN-g (gamma) BV711 (Clone B27) BD Biosciences Cat # 564039; RRID: AB_2738557 Human CD107a (LAMP-1) (Clone H4A3) BD Biosciences Cat # 561343; RRID: AB_10644020 Human PD-L1 (CD273, B7-H1) BV421 (Clone MIH1) BD Biosciences Cat # 563738; RRID: AB_2738396 Human CD73 PE (Clone REA804) Miltenyi Biotec Cat # 130-112-060; RRID: AB_2659164 Human NKG2D (CD314) PE (Clone 1D11) BD Bioscience Cat # 557940; RRID: AB_396951 Human CD25 PE-Cy7 (Clone M-A251) BD Biosciences Cat # 557741; RRID: AB_396847 Human FoxP3 APC (Clone FJK-16s) Thermo Fisher Scientific Cat # 17-5773-82; RRID: AB_469457 Human purified anti-IL-10 (Clone JES3-9D7) BioLegend Cat # 501401; RRID: AB_315167 Human purified anti-IL-10R (Clone 3F9) BioLegend Cat # 308802; RRID: AB_314734 Isotype control (for human IL-10) (Clone eBRG1) BioLegend Cat # 16-4301-85; RRID: AB_470154 Isotype control (for human IL-10R) (Clone RTK2758) Thermo Fisher Scientific Cat # 400516; RRID: AB_2861020 Mouse CD3 FITC (Clone REA641) Miltenyi Biotec Cat # 130-119-798; RRID: AB_2751851 Mouse CD73 AF647 (Clone TY/23) BD Biosciences Cat # 561543; RRID: AB_10896329 Mouse CD69 APC-efluor780 (Clone H1.2F3) Thermo Fisher Scientific Cat # 47-0691-82; RRID: AB_2573966 Mouse NK1.1 APC (Clone PK136) BioLegend Cat # 108710; RRID: AB_313397 Mouse NK1.1 PerCP-Vio700 (Clone PK136) Miltenyi Biotec Cat # 130-117-773; RRID: AB_2728038 (Continued on next page) Cell Reports 39, 110847, May 24, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse NK1.1 PerCP/Cyanine 5.5 (Clone PK136) BioLegend Cat # 108727; RRID: AB_2132706 Mouse CD11c APC-eFluor 780 (Clone N418) Thermo Fisher Scientific Cat # 47-0114-82; RRID: AB_1548652 Mouse CD11b BV650 (Clone M1/70) BD Biosciences Cat # 563402; RRID: AB_2738184 Mouse Ly6c PerCP/Cyanine 5.5 (Clone HK1.4) Biolegend Cat # 128012; RRID: AB_1659241 Mouse CD19 PE-Vio770 (Clone REA749) Miltenyi Biotec Cat # 130-111-885; RRID: AB_2655831 Mouse CD86 BV421 (Clone GL1) BD Biosciences Cat # 564198; RRID: AB_2738663 Mouse MHC II (I-Ab) biotin (Clone 25-9-17) BD Biosciences Cat # 553604; RRID: AB_394948 Mouse MHC II (I-A/I-E) (Clone M5/114.15.2) Thermo Fisher Scientific Cat # 56-5321-82; RRID: AB_494009 Mouse CD8a BV605 (Clone 53–6.7) BioLegend Cat # 100744; RRID: AB_2562609 Mouse IFN-gamma BV650 (Clone XMG1.2) BioLegend Cat # 505831; RRID: AB_11142685 Mouse IFN-gamma PE-CF594 (Clone XMG1.2) BD Biosciences Cat # 562303; RRID: AB_11153140 Mouse CD45.1 PerCP-Cy5.5 (Clone A20) Thermo Fisher Scientific Cat # 45-0453-82; RRID: AB_1107003 Mouse PD-L1 (CD274, B7-H1) PE (Clone 10F.9G2) BioLegend Cat # 124308; RRID: AB_2073556 Mouse CD107a (LAMP-1) APC (Clone 1D4B) Biolegend Cat # 121613; RRID: AB_2134486 Mouse Perforin PE (Clone S16009B) BioLegend Cat # 154405; RRID: AB_2721640 Mouse TLR4 (CD284) PE (Clone SA15-21) BioLegend Cat #: 145403; RRID: AB_2561873 Mouse TLR9 (CD289) FITC (Clone M9.D6) Thermo Fisher Scientific Cat # 11-9093-80; RRID: AB_465443 Bacterial and virus strains VSV-Dm51 Dr. Douglas Mahoney N/A Biological samples Healthy human peripheral blood mononuclear cells Canadian Blood Services Blood4Research program Chemicals, peptides, and recombinant proteins Protein Assay Dye Reagent Concentrate Bio-Rad Cat # 5000006 Pierce Chromogenic Endotoxin Quant Kit Thermo Fisher Scientific Cat # A39552 NK MACS Medium (human NK cell expansion medium) Miltenyi Biotec Cat # 130-114-429 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat # 2430054 Roswell Park Memorial Institute 1649 Medium (RPMI) Thermo Fisher Scientific Cat # 11875093 HEPES buffer Thermo Fisher Scientific Cat # 15630106 Minimum Essential Medium a (MEM-a) Thermo Fisher Scientific Cat # 12571063 Horse Serum, heat inactivated Thermo Fisher Scientific Cat # 26050070 Myo-Inositol Milipore Sigma Cat # I7508 b-mercaptoethanol Thermo Fisher Scientific Cat # 21985023 Folic acid Milipore Sigma Cat # F8758 Sodium pyruvate Thermo Fisher Scientific Cat # 25000CI (Continued on next page) e2 Cell Reports 39, 110847, May 24, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: organisms/strains Mouse: C57BL/6NCrl Charles River Laboratories https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Mouse: IL-10-IRES-eGFP knock-in (IL-10GFP or tiger [interleukin-ten ires gfo-enhanced reporter]) Dr. Richard A. Flavell, via Dr. Jun Wang https://doi.org/10.1016/j.immuni.2006.09.013 Oligonucleotides TLR 9 agonist CpG DNA Invivogen Cat # tlrl-1668 Software and algorithms FlowJo Version 10.7.1 BD Biosciences https://www.flowjo.com/ Prism Version 9.1.2 GraphPad https://www.graphpad.com/scientific-software/ prism/ Reactome Pathway Browser 3.7 Reactome https://reactome.org/; RRID: SCR_003485 Uniprot Universal Protein Resource https://www.uniprot.org/; RRID: SCR_002380

    Modification:

    Article Title: Mitochondrial damage-associated molecular patterns trigger arginase-dependent lymphocyte immunoregulation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Human CD56 PE-Vio615 (Clone REA196) Miltenyi Biotec Cat # 130-114-550; RRID: AB_2726696 Human CD56 FITC (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726695 Human CD56 PE (Cone REA196) Miltenyi Biotec Cat # 130-113-312; RRID: AB_2726090 Human CD56 PerCP-Vio 700 (Cone REA196) Miltenyi Biotec Cat # 130-114-551; RRID: AB_2733038 Human CD3 (CD3ε) APC (Clone REA613) Miltenyi Biotec Cat # 130-113-697; RRID: AB_2726238 Human CD3 (CD3ε) BV650 (Clone UCHT1) BD Biosciences Cat # 563851; RRID: AB_2744391 Human CD8 BV650 (Clone RPA-T8) BD Biosciences Cat # 563821; RRID: AB_2744462 Human CD4 PerCP-Cy5.5 (Clone SK3 [also known as Leu3a]) BD Biosciences Cat # 566321; RRID: AB_2739683 Human CD69 AF700 (Clone FN50) BD Biosciences Cat # 560739; RRID: AB_1727505 Human CD69 BV421 (Clone FN50) BD Biosciences Cat # 562884; RRID: AB_2687422 Human IFN-g (gamma) BV711 (Clone B27) BD Biosciences Cat # 564039; RRID: AB_2738557 Human CD107a (LAMP-1) (Clone H4A3) BD Biosciences Cat # 561343; RRID: AB_10644020 Human PD-L1 (CD273, B7-H1) BV421 (Clone MIH1) BD Biosciences Cat # 563738; RRID: AB_2738396 Human CD73 PE (Clone REA804) Miltenyi Biotec Cat # 130-112-060; RRID: AB_2659164 Human NKG2D (CD314) PE (Clone 1D11) BD Bioscience Cat # 557940; RRID: AB_396951 Human CD25 PE-Cy7 (Clone M-A251) BD Biosciences Cat # 557741; RRID: AB_396847 Human FoxP3 APC (Clone FJK-16s) Thermo Fisher Scientific Cat # 17-5773-82; RRID: AB_469457 Human purified anti-IL-10 (Clone JES3-9D7) BioLegend Cat # 501401; RRID: AB_315167 Human purified anti-IL-10R (Clone 3F9) BioLegend Cat # 308802; RRID: AB_314734 Isotype control (for human IL-10) (Clone eBRG1) BioLegend Cat # 16-4301-85; RRID: AB_470154 Isotype control (for human IL-10R) (Clone RTK2758) Thermo Fisher Scientific Cat # 400516; RRID: AB_2861020 Mouse CD3 FITC (Clone REA641) Miltenyi Biotec Cat # 130-119-798; RRID: AB_2751851 Mouse CD73 AF647 (Clone TY/23) BD Biosciences Cat # 561543; RRID: AB_10896329 Mouse CD69 APC-efluor780 (Clone H1.2F3) Thermo Fisher Scientific Cat # 47-0691-82; RRID: AB_2573966 Mouse NK1.1 APC (Clone PK136) BioLegend Cat # 108710; RRID: AB_313397 Mouse NK1.1 PerCP-Vio700 (Clone PK136) Miltenyi Biotec Cat # 130-117-773; RRID: AB_2728038 (Continued on next page) Cell Reports 39, 110847, May 24, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse NK1.1 PerCP/Cyanine 5.5 (Clone PK136) BioLegend Cat # 108727; RRID: AB_2132706 Mouse CD11c APC-eFluor 780 (Clone N418) Thermo Fisher Scientific Cat # 47-0114-82; RRID: AB_1548652 Mouse CD11b BV650 (Clone M1/70) BD Biosciences Cat # 563402; RRID: AB_2738184 Mouse Ly6c PerCP/Cyanine 5.5 (Clone HK1.4) Biolegend Cat # 128012; RRID: AB_1659241 Mouse CD19 PE-Vio770 (Clone REA749) Miltenyi Biotec Cat # 130-111-885; RRID: AB_2655831 Mouse CD86 BV421 (Clone GL1) BD Biosciences Cat # 564198; RRID: AB_2738663 Mouse MHC II (I-Ab) biotin (Clone 25-9-17) BD Biosciences Cat # 553604; RRID: AB_394948 Mouse MHC II (I-A/I-E) (Clone M5/114.15.2) Thermo Fisher Scientific Cat # 56-5321-82; RRID: AB_494009 Mouse CD8a BV605 (Clone 53–6.7) BioLegend Cat # 100744; RRID: AB_2562609 Mouse IFN-gamma BV650 (Clone XMG1.2) BioLegend Cat # 505831; RRID: AB_11142685 Mouse IFN-gamma PE-CF594 (Clone XMG1.2) BD Biosciences Cat # 562303; RRID: AB_11153140 Mouse CD45.1 PerCP-Cy5.5 (Clone A20) Thermo Fisher Scientific Cat # 45-0453-82; RRID: AB_1107003 Mouse PD-L1 (CD274, B7-H1) PE (Clone 10F.9G2) BioLegend Cat # 124308; RRID: AB_2073556 Mouse CD107a (LAMP-1) APC (Clone 1D4B) Biolegend Cat # 121613; RRID: AB_2134486 Mouse Perforin PE (Clone S16009B) BioLegend Cat # 154405; RRID: AB_2721640 Mouse TLR4 (CD284) PE (Clone SA15-21) BioLegend Cat #: 145403; RRID: AB_2561873 Mouse TLR9 (CD289) FITC (Clone M9.D6) Thermo Fisher Scientific Cat # 11-9093-80; RRID: AB_465443 Bacterial and virus strains VSV-Dm51 Dr. Douglas Mahoney N/A Biological samples Healthy human peripheral blood mononuclear cells Canadian Blood Services Blood4Research program Chemicals, peptides, and recombinant proteins Protein Assay Dye Reagent Concentrate Bio-Rad Cat # 5000006 Pierce Chromogenic Endotoxin Quant Kit Thermo Fisher Scientific Cat # A39552 NK MACS Medium (human NK cell expansion medium) Miltenyi Biotec Cat # 130-114-429 Dulbecco’s Modified Eagle Medium (DMEM) Thermo Fisher Scientific Cat # 2430054 Roswell Park Memorial Institute 1649 Medium (RPMI) Thermo Fisher Scientific Cat # 11875093 HEPES buffer Thermo Fisher Scientific Cat # 15630106 Minimum Essential Medium a (MEM-a) Thermo Fisher Scientific Cat # 12571063 Horse Serum, heat inactivated Thermo Fisher Scientific Cat # 26050070 Myo-Inositol Milipore Sigma Cat # I7508 b-mercaptoethanol Thermo Fisher Scientific Cat # 21985023 Folic acid Milipore Sigma Cat # F8758 Sodium pyruvate Thermo Fisher Scientific Cat # 25000CI (Continued on next page) e2 Cell Reports 39, 110847, May 24, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: organisms/strains Mouse: C57BL/6NCrl Charles River Laboratories https://www.criver.com/products-services/ find-model/c57bl6-mouse?region=24 Mouse: IL-10-IRES-eGFP knock-in (IL-10GFP or tiger [interleukin-ten ires gfo-enhanced reporter]) Dr. Richard A. Flavell, via Dr. Jun Wang https://doi.org/10.1016/j.immuni.2006.09.013 Oligonucleotides TLR 9 agonist CpG DNA Invivogen Cat # tlrl-1668 Software and algorithms FlowJo Version 10.7.1 BD Biosciences https://www.flowjo.com/ Prism Version 9.1.2 GraphPad https://www.graphpad.com/scientific-software/ prism/ Reactome Pathway Browser 3.7 Reactome https://reactome.org/; RRID: SCR_003485 Uniprot Universal Protein Resource https://www.uniprot.org/; RRID: SCR_002380



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